CLINICAL RESEARCH
The role of FokI polymorphism of vitamin D receptor gene and vitamin D level in multidrug-resistant tuberculosis occurrence in Medan city, Indonesia
Submission date: 2018-04-06
Final revision date: 2018-08-07
Acceptance date: 2018-09-28
Publication date: 2018-12-17
Arch Med Sci Civil Dis 2018;3(1):153-157
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Multidrug-resistant tuberculosis (MDR-TB) has become a new problem in the world. Many factors have been associated with MDR-TB occurrence. Some studies have demonstrated a role of vitamin D level and vitamin D receptor (VDR) gene polymorphism in MDR-TB. The aim of this study is to explore the role of FokI polymorphisms of the VDR gene and vitamin D level in MDR-TB occurrence in Medan city, Indonesia.
Material and methods:
This is a case-control study. Cases were 43 MDR-TB patients from the MDR-TB polyclinic Adam Malik Hospital in Medan, Indonesia. The control group comprised 56 new pulmonary TB cases with positive AFB sputum smear. Patients who were HIV positive, known to have diabetes mellitus or other severe disease, taking immunosuppressive drugs and vitamin D were excluded for both groups. Genetic polymorphisms of the VDR gene were analyzed using PCR-RFLP. Vitamin D level was analyzed using the ELISA procedure.
Results:
The frequencies of FokI genotypes were FF 39.5%, Ff 53.5%, ff 7.0% for MDR-TB patients and FF 39.3%, Ff 46.4.% and ff 14.3% for controls. There was no significant association between FokI genotype of the VDR gene and MDR-TB. Vitamin D level in MDR-TB was 53.5% sufficient, 39.5% insufficient and 7.0% deficient. Vitamin D level was 41.1% sufficient, 46.4% insufficient and 12.5% deficient in TB non-MDR. There was no significant association between vitamin D level and MDR-TB.
Conclusions:
No role of FokI polymorphism of the VDR gene and vitamin D level in MDR-TB occurrence was found.
Multidrug-resistant tuberculosis (MDR-TB) has become a new problem in the world. Many factors have been associated with MDR-TB occurrence. Some studies have demonstrated a role of vitamin D level and vitamin D receptor (VDR) gene polymorphism in MDR-TB. The aim of this study is to explore the role of FokI polymorphisms of the VDR gene and vitamin D level in MDR-TB occurrence in Medan city, Indonesia.
Material and methods:
This is a case-control study. Cases were 43 MDR-TB patients from the MDR-TB polyclinic Adam Malik Hospital in Medan, Indonesia. The control group comprised 56 new pulmonary TB cases with positive AFB sputum smear. Patients who were HIV positive, known to have diabetes mellitus or other severe disease, taking immunosuppressive drugs and vitamin D were excluded for both groups. Genetic polymorphisms of the VDR gene were analyzed using PCR-RFLP. Vitamin D level was analyzed using the ELISA procedure.
Results:
The frequencies of FokI genotypes were FF 39.5%, Ff 53.5%, ff 7.0% for MDR-TB patients and FF 39.3%, Ff 46.4.% and ff 14.3% for controls. There was no significant association between FokI genotype of the VDR gene and MDR-TB. Vitamin D level in MDR-TB was 53.5% sufficient, 39.5% insufficient and 7.0% deficient. Vitamin D level was 41.1% sufficient, 46.4% insufficient and 12.5% deficient in TB non-MDR. There was no significant association between vitamin D level and MDR-TB.
Conclusions:
No role of FokI polymorphism of the VDR gene and vitamin D level in MDR-TB occurrence was found.
REFERENCES (22)
1.
World Health Organization. Multidrug and extensively drug-resistant TB (MDR/XDR-TB): 2010 global report on surveillance and response. WHO Library Cataloguing-in- Publication Data. WHO/HTM/TB/2010.
2.
World Health Organization. Global tuberculosis report 2014. Geneva, Switzerland: WHO Press 2015.
3.
Liu PT, Stenger S, Tang DH, et al. Cutting edge: vitamin D-mediated human antimicrobial activity against Mycobacterium tuberculosis is dependent on the induction of cathelicidin. J Immunol 2007; 179: 2060-3.
4.
Chun RF, Adams JS, Hewison M. Back to the future: a new look at ‘old’ vitamin D. J Endocrinol 2008; 198: 261-9.
5.
Zmuda JM, Cauley JA, Ferrell RE. Molecular epidemiology of vitamin D receptor gene variants. Epidemiol Rev 2000; 22: 203-17.
6.
Arai H, Myamoto K, Taketani Y, et al. A vitamin D receptor gene polymorphism in the translation initiation codon: effect on protein and relation to bone mineral density in Japanese women. J Bone Miner Res 1997; 12: 915-21.
7.
Jurutka PW, Remus LS, Whitfield GK, et al. The polymorphic N terminus in human vitamin D receptor isoform influences transcriptional activity by modulating interaction with transcription factor IIB. Mol Endocrinol 2000; 14: 401-20.
8.
Thaha ILM, Yusuf I, Hadju V, Massi N. Polimorfisme ApaI, FokI, BsmI gen receptor vitamin D terhadap Kejadian Multidrug Resisten Tuberkulosis (MDR-TB). JST Kesehatan 2012; 2: 390-7.
9.
Rathored J, Sharma SK, Singh B, et al. Low serum vitamin D in North Indian multi-drug resistant pulmonary tuberculosis patients: the role of diet and sunlight. J Bacteriol Parasitol 2015; 6: 4.
10.
Sinaga BYM, Amin M, Siregar Y, Sarumpaet S. Correlation between vitamin D receptor gene FOKI and BSMI polymorphims and the susceptibility to pulmonary tuberculosis in an Indonesian Batak-ethnic population. Indon J Intern Med Acta Med Indon 2014; 46: 275-82.
11.
Nnoaham KE, Clarke A. Low serum vitamin D levels and tuberculosis: a systematic review and meta-analysis. Int J Epidemiol 2008; 37: 113-9.
12.
Huang S, Wang X, Liu Z, et al. Vitamin D deficiency and the risk of tuberculosis: a meta-analysis. Drug Des Dev Ther 2017; 11: 91-102.
13.
Rathored J, Sharma SK, Singh B, et al. Risk and outcome of multidrug-resistant tuberculosis: vitamin D receptor polymorphisms and serum 25(OH)D. Int J Tuberc Lung Dis 2012; 16: 1522-8.
14.
Iftikhar R, Kamran SM, Qadir A, Haider E, Usman HB. Vitamin D deficiency in patients with tuberculosis. J Coll Physicians Surg Pak 2013; 23: 780-3.
15.
Herlina N, Sinaga BYM, Siagian P, Mutiara E. Serum vitamin D level and susceptibility to multidrug-resistant tuberculosis among household contacts. IOP Conference Series: Earth and Environmental Science 2018; 125(1).
16.
Zittermann A. Vitamin D in preventive medicine: are we ignoring the evidence? Br J Nutr 2003; 89: 552-72.
17.
Uitterlinden AG, Fanf Y, van Meurs JBJ, Pols HAP, van Leeuwen JPTM. Genetics and biology of vitamin D receptor polymorphisms. Gene 2004; 338: 143-56.
18.
Faustini A, Hall AJ, Perucci CA. Risk factors for multi drug resistant tuberculosis in Europe: a systematic review. Thorax 2006; 61: 158-63.
19.
World Health Organization. Multi Drug – Resistant Tuberculosis (MDR-TB) 2016 Update. WHO 2016.
20.
Velez DR, Hulme WF, Myers JL, et al. NOS2A, TLR4, and IFNGR1 interaction influence pulmonary tuberculosis susceptibility in African-Americans. Hum Genet 2009; 126: 43-53.
21.
Van Crevel R, Parwati I, Sahiratmadja E, et al. Infection with Mycobacterium tuberculosis Beijing genotype strains is associated with polymorphisms in SLCHA1/NRAMP in Indonesian patients with tuberculosis. J Infect Dis 2009; 200: 1671-4.
22.
Sari DK, Damanik HA, Lipoeto NI, Lubis Z. Is micro evolution in tropical country women resulting low 25(OH)D level? A cross sectional study in Indonesia. J Nutr Food Sci 2013; 4: 246.
| ISSN: | 2451-0637 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
