Study of expression of two transient receptor potential (TRP) channels, TRPC5 and TRPC6, in children with infantile hypertrophic pyloric stenosis
More details
Hide details
Submission date: 2016-06-26
Acceptance date: 2016-08-14
Publication date: 2016-08-23
Arch Med Sci Civil Dis 2016;1(1):66-69
Introduction: The transient receptor potential (TRP) channel, so named after the role they play in Drosophila phototransduction, takes a prominent position among these sensors and transducers in the digestive system. However, the roles of TRP channels in digestive function are incompletely understood. Changes in TRP channel expression or function are associated with a variety of diseases. The aim of this study was to analyze the expression of TRPC5 and TRPC6 by immunohistochemistry in the pyloric muscle in children with infantile hypertrophic pyloric stenosis (IHPS).
Material and methods: Fifty-three muscle biopsies were obtained at the time of pyloromyotomy. The control group consisted of 10 children. Specimens were evaluated by routine histopathological methods and by immunohistochemistry using monoclonal mouse anti-TRPC5 or -TRPC6 antibodies. Cells showing positive reaction were counted in five random 200× high-power fields.
Results: Immunohistochemical studies showed higher expression of both receptors in the muscularis propria and in the ganglia in children with infantile hypertrophic pyloric stenosis compared with the control group. The result for TRPC5 was statistically significant.
Conclusions: These results show a local increase in the expression of TRPC5 and TRPC6 in the hypertrophic pyloric muscle in children with infantile hypertrophic pyloric stenosis, which may have implications for the pathogenesis of the disease.
Holzer P. Transient receptor potential (TRP) channels as drug targets for diseases of the digestive system. Pharmacol Ther 2011; 131: 142-70.
Abramowitz J, Birnbaumer L. Physiology and pathophysiology of canonical transient receptor potential channels. FASEB J 2009; 23: 297-328.
MacMahon B. The continuing enigma of pyloric stenosis of infancy: a review. Epidemiology 2006; 17: 195-201.
Murtagh K, Perry P, Corlett M, Fraser I. Infantile hypertrophic pyloric stenosis. Dig Dis 1992; 10: 190-8.
Everett KV, Chioza BA, Georgoula C, Reece A, Gardiner RM, Chung EM. Infantile hypertrophic pyloric stenosis: evaluation of three positional candidate genes, TRPC1, TRPC5 and TRPC6, by association analysis and re-sequencing. Hum Genet 2009; 126: 819-31.
Huang CL. The transient receptor potential superfamily of ion channels. J Am Soc Nephrol 2004; 15: 1690-9.
Dietrich A, Chubanov V, Kalwa H, Rost BR, Gudermann T. Cation channels of the transient receptor potential superfamily: their role in physiological and pathophysiological processes of smooth muscle cells. Pharmacol Ther 2006; 112: 744-60.
Moller CC, Mangos S, Drummond IA, Reiser J. Expression of trpC1 and trpC6 orthologs in zebrafish. Gene Expr Patterns 2008; 8: 291-6.
Spassova MA, Hewavitharana T, Xu W, SoboloV J, Gill DL. A common mechanism underlies stretch activation and receptor activation of TRPC6 channels. Proc Natl Acad Sci USA 2006; 103: 16586-91.
Bae YM, Kim A, Lee YJ, et al. Enhancement of receptor-operated cation current and TRPC6 expression in arterial smooth muscle cells of deoxycorticosterone acetate-salt hypertensive rats. J Hypertens 2007; 25: 809-17.
Kuwahara K, Wang Y, McAnally J, et al. TRPC6 fulfills a calcineurin signaling circuit during pathologic cardiac remodeling. J Clin Invest 2006; 116: 3114-26.
Lin MJ, Leung GP, Zhang WM, et al. Chronic hypoxia-induced upregulation of store-operated and receptor-operated Ca2+channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension. Circ Res 2004; 95: 496-505.
Wang J, Weigan D, Lu W, Sylvester JT, Semenza GL, Shimoda LA. Hypoxia inducible factor 1 mediates hypoxia-.
induced TRPC expression and elevated intracellular Ca2 in pulmonary arterial smooth muscle cells. Circ Res 2006; 98: 1528-37.
Yu Y, Fantozzi I, Remillard CV, et al. Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension. Proc Natl Acad Sci USA 2004; 101: 13861-6.
Everett KV, Chioza BA, Georgoula C, et al. High-density SNP-based linkage analysis of infantile hypertrophic pyloric stenosis identifies loci on chromosomes 11q14-q22 and Xq23. Am J Hum Genet 2008; 82: 756-62.
Brouwers AG, Waals-van de Wal CM. Hypertrophic pyloric stenosis and pulmonary hypertension in a neonate. A common mechanism? Acta Paediatr 2009; 98: 1064-5.
Lee YM, Kim BJ, Kim HJ, et al. TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach. Am J Physiol Gastrointest Liver Physiol 2003; 284: G604-16.
Bush EW, Hood DB, Papst PJ, et al. Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling. J Biol Chem 2006; 281: 33487-96.
Yoshida T, Inoue R, Morii T, et al. Nitric oxide activates TRP channels by cysteine S-nitrosylation. Nat Chem Biol 2006; 2: 596-607.
Journals System - logo
Scroll to top